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Four cell linesuggs pas cher(CMK, HEL, MONO MAC 1 and U937) demonstrated interstitial or terminal deletions, with breakpoints mapping centromeric to marker D20174, which is located at the proximal end of the CDR defined using MPD patient samples (Figure 2). A mono allelic pattern was found at all 20q and 20p loci in the KBM 5 and KBMugg enfant pas cher7 cell lines and for all but one marker in MEG O1 and MOLM 1 cells, suggesting reduction to monosomy for chromosome 20. Microsatellite instability (indicated by the laddering of PCR products) was detected at many different loci in MKB 1 and RC 2A cells, whereas it was occasionally observed in KCL 22 cellsair max 90 pas cher(data not shown).PCR based amplification failed repeatedly at D20S110 in CMK cells, at D20S836 in U937 cells, and at D20S881 in MEG O1 cells, however new sets of primers in nested or semi nested amplification reactions (see Materials and methods), led to successful amplification at D20S110 and D20S836; howeverlongchamp pas cheramplification at D20881 failed repeatedly, suggesting the presence of a homozygous deletion at this locus, which was confirmed by Southern blot analysis (Figure 3).To identify additional homozygous deletions or intragenic rearrangements contained within the CDR, Southern blots containing EcoRI and HindIIIsac longchamp pliage pas cherdigested genomic DNA from the eight mono allelic cell lines were probed using a set of 37 cDNA clones (corresponding to 27 UniGenes and 10 anonymous ESTs) characterized for hybridization to single copy sequences and correct chromosomal mapping (Table 1).

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